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. 2020 Feb 18;10:2843. doi: 10.1038/s41598-020-59724-7

Table 1.

Sequence of primers used for Q-PCR analysis.

Gene Nucleotide sequence (5′-3′) Accession number
Endogenous genes
β- ACTIN

Forward CGCACCACTGGCATTGTCAT

Reverse TCCAAGGCGGACGTAGCAGAG

NM_173979.3
GAPDH

Forward CCTTCATTGACCTTCACTACATGGTCTA

Reverse CCTTCATTGACCTTCACTACATGGTCTA

NM_001034034.2
Lymphocyte activation genes
CD4

Forward TTCCTTCCCACTCACCTTCG

Reverse ATCTTGTTCACCTTCACCTCTC

XM_024991418.1
CD8

Forward AAGGCATACCACAAGGGTTATC

Reverse CACAGCCAGGTTCTGAGAATAG

NM_174015.1
CD25

Forward GGAAAGCCCTAACACTGATGTA

Reverse AGAGGCTTGGAAAGGACTTATG

NM_174358.2
CD62L

Forward TACGAAAGGACGGAGCAAAG

Reverse GAGCATAATCCAGACCCACAG

NM_174182.1
CD69

Forward GGGTCCATTCAAGTTCCTATCC

Reverse TGTACTGGCCCACTGATAGA

NM_174014.2
Proinflammatory cytokine genes
IL2

Forward GGGAACACAATGAAAGAAGTGAAG

Reverse GAGCTTGAGGTTCTCAGGATTT

NM_180997.2
IFNɣ

Forward AACACAGGAGCTACCGATTTC

Reverse AAGCCCACAGAGCAGTAAAG

NM_174086.1
TNFɑ

Forward GCCAACTCCCTCTGTTTATGT

Reverse GACACCTTGACCTCCTGAATAA

NM_173966.3
CCL2

Forward TCGCCTGCTGCTATACATTC

Reverse CACAGCCTCTTTAGGACACTT

NM_174006.2
CCL5 Forward CCTGCTGCTTTGCCTATATCT NM_175827.2
Reverse GCGCTTCTTCCTGGTGATAA
CXCL3 Forward ACATAACCCAGTCCTGATTGTT NM_001046513.2
Reverse CACAGAGCCTGGCACTTTAT