Table 1.
Gene | Nucleotide sequence (5′-3′) | Accession number |
---|---|---|
Endogenous genes | ||
β- ACTIN |
Forward CGCACCACTGGCATTGTCAT Reverse TCCAAGGCGGACGTAGCAGAG |
NM_173979.3 |
GAPDH |
Forward CCTTCATTGACCTTCACTACATGGTCTA Reverse CCTTCATTGACCTTCACTACATGGTCTA |
NM_001034034.2 |
Lymphocyte activation genes | ||
CD4 |
Forward TTCCTTCCCACTCACCTTCG Reverse ATCTTGTTCACCTTCACCTCTC |
XM_024991418.1 |
CD8 |
Forward AAGGCATACCACAAGGGTTATC Reverse CACAGCCAGGTTCTGAGAATAG |
NM_174015.1 |
CD25 |
Forward GGAAAGCCCTAACACTGATGTA Reverse AGAGGCTTGGAAAGGACTTATG |
NM_174358.2 |
CD62L |
Forward TACGAAAGGACGGAGCAAAG Reverse GAGCATAATCCAGACCCACAG |
NM_174182.1 |
CD69 |
Forward GGGTCCATTCAAGTTCCTATCC Reverse TGTACTGGCCCACTGATAGA |
NM_174014.2 |
Proinflammatory cytokine genes | ||
IL2 |
Forward GGGAACACAATGAAAGAAGTGAAG Reverse GAGCTTGAGGTTCTCAGGATTT |
NM_180997.2 |
IFNɣ |
Forward AACACAGGAGCTACCGATTTC Reverse AAGCCCACAGAGCAGTAAAG |
NM_174086.1 |
TNFɑ |
Forward GCCAACTCCCTCTGTTTATGT Reverse GACACCTTGACCTCCTGAATAA |
NM_173966.3 |
CCL2 |
Forward TCGCCTGCTGCTATACATTC Reverse CACAGCCTCTTTAGGACACTT |
NM_174006.2 |
CCL5 | Forward CCTGCTGCTTTGCCTATATCT | NM_175827.2 |
Reverse GCGCTTCTTCCTGGTGATAA | ||
CXCL3 | Forward ACATAACCCAGTCCTGATTGTT | NM_001046513.2 |
Reverse CACAGAGCCTGGCACTTTAT |