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. 2020 Feb 18;11(2):125. doi: 10.1038/s41419-020-2324-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Laser scanning confocal microscopy was used to observe SESN2 and ER in DC2.4 cells. It was shown by anti-SESN2 antibodies (green) and ER tracker (red) using indirect immunofluorescence and confocal microscopy. b It was observed by anti-SESN2 antibodies (red) and ATF4 (green) using indirect immunofluorescence and confocal microscopy. c It was shown by anti-SESN2 antibodies (red) and GRP78 (green) using immunofluorescence and confocal microscopy. d Immunoblot analysis of ATF4 in SESN2 was immunoprecipitated in total cell lysate. Negative control was using control agarose resin. Scale bar = 25 μm, scale bar = 10 μm, n = 3 per group. Its co-localization was shown in saffron yellow (DAPI stained nucleus, blue).