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. 2020 Feb 18;11(2):125. doi: 10.1038/s41419-020-2324-4

Fig. 5. SESN2 protected DC2.4 cells against ERS-related apoptosis triggered by TM.

Fig. 5

DC2.4 cells were treated with various dosages of TM (0.25, 0.5, 1, and 100 ng/ml) for 48 h. Cells cultured for 48 h without TM were used as the controls. a PE-Annexin-V and 7-AAD were used to stain the treated DC2.4 cells and subjected to flow cytometry to assess cell apoptosis with different dosages of TM for 48 h. b SESN2 expression was determined by Western blotting. c After modulated SESN2 expression, PE-Annexin V and 7-AAD were used to stain the treated DC2.4 cells and flow cytometry was used to assess cell apoptosis after stimulated with 2 μg/ml TM for 48 h. d Expressions of SESN2 and CHOP were measured as described in the section of methods following treatment with 2 μg/ml TM for 48 h. β-actin served as the internal standard. Data of three independent experiments were presented as the mean ± SD. Statistical significance: *P < 0.05 versus the scramble group; #P < 0.05 versus the scramble group treated with HMGB1.