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. 2020 Feb 18;11(2):125. doi: 10.1038/s41419-020-2324-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Mice underwent a sham procedure or CLP. a SESN2 expression was determined by Western blotting at 24 h after after CLP. b PE-Annexin-V and 7-AAD were used to stain DCs and subjected to flow cytometry to assess cell apoptosis at 24 h after CLP in vivo. c Expressions of cleaved-caspase-3, Bcl-2, and Bax were measured as described in the section of methods after CLP procedure. d In the sesn2^−/− CLP group, the morphologic alteration of ER in each cell was evaluated by immunofluorescence staining and the extent of the ER morphologic change was compared with the WT CLP group (×1200). ER was detected with ER-tracker red. Levels of GRP78, ATF4, and CHOP were assessed by Western blotting. β-actin served as the internal standard. e The 24-h survival rate of mice in the sesn2^−/− CLP group was markedly lower than that in the WT CLP group. Data of three independent experiments were presented as the mean ± SD, n = 6 per sham group, n = 11–13 per CLP group. Statistical significance: *P < 0.05 for in vivo comparison of the sesn2^−/− CLP group versus the WT CLP group.