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. 2020 Feb 18;9(2):24. doi: 10.1038/s41389-020-0209-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Left: Immunoblot analyses of the Proteome Profiler Human Phospho-Kinase Array comparing OVCAR-3 cells with and without PDK1 overexpression. Middle: The spot pixel density on the array was quantified using ImageJ software. The spot targets with >1.5-fold changes between the two groups were recorded. The mean pixel density of p-JNK1/2/3 (T183/Y185 and T221/Y223), p-c-Jun (S63), p-PCL-γ1 (Y783), and p-Stat3 (Y705) in OVCAR-3 cells with stable PDK1 overexpression. Right: Immunoblot analyses of p-JNK, p-c-Jun, and JNK in PDK1-overexpressing OVCAR-3 and PDK1-depleted A2780CP (shPDK1-316) cells. b IL-8 mRNA expression and protein secretion in control or PDK1-overexpressing OVCAR-3 cells treated with DMSO (vehicle) or the JNK inhibitor, SP600125, determined via qPCR (left) and ELISA (right), presented as fold change relative to controls; bars: mean ± SD of three experiments; *P < 0.05; Mann–Whitney test. c Upper: In vitro migration and invasion assays with control or PDK1-overexpressing OVCAR-3 cells treated with DMSO or SP600125. Cell migration and invasion are presented as a percentage of controls; bars: mean ± SD of three experiments; *P < 0.05; Mann–Whitney test. Lower: Capillary tube formation by HUVECs treated with conditioned medium from control or PDK1-overexpressing OVCAR-3 cells subjected to DMSO or SP600125 treatment.