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. 2020 Feb 18;10:2813. doi: 10.1038/s41598-020-59690-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Project workflow. Discovery and technical validation set: CRC and normal DNA samples collected for the study of NGS. Bioinformatic analysis set: Bioinformatic analysis among Methylation and Expression database and current expriment for detecting robust hypo- hyper methylation genes. Biological validation and tissue expression set: CRC and normal FFPE samples used for Biological validation and candidate gene expression by real time PCR. Plasma study sets: CRC and normal plasma samples used for methylation study of candidate gene.