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. 2019 Dec 17;122(4):517–527. doi: 10.1038/s41416-019-0681-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Cells were treated with docetaxel and mebendazole at the indicated concentrations for 48 h. Each plate contained 8 × 8-dose matrix blocks with serial twofold dilutions of docetaxel and 1.333-fold dilutions of mebendazole. Percentage growth inhibition was calculated for each drug combination using the CellTiter-Glo Assay (Promega). The combination index (CI) was calculated using CompuSyn software, where < 1 indicates synergism, = 1 is an additive effect and > 1 indicates antagonism. The percentage and colour codes for growth inhibition and CI scales are shown on the left. b SP1 (upper panel) and PC3 (lower panel) cells were synchronised using a double-thymidine block before being drug treated, stained with propidium iodide (PI) and analysed using flow cytometry. Both drugs individually cause a G2/M arrest, and the block is further increased when the cells are treated with both drugs. n = 3, a representative image is shown. c Percentages of cells in each phase of the cell cycle were calculated and plotted. n = 3, mean values ± SD are shown, analysed by two-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, ***p < 0.0001. d Cells were treated with different drug combinations, stained with annexin V and propidium iodide and analysed by flow cytometry (left panel, a representative image is shown). The percentage of cells stained with annexin V is shown in the right panel. n = 3 (independent experiments), mean values ± SD are shown, analysed by two-way ANOVA with Sidak’s multiple comparisons test, *p < 0.01.