Fig. 4.

Combining docetaxel and mebendazole perturbs microtubule dynamics by reducing microtubule assembly speed. a PC3 and b SP1 cells were seeded on coverslips and treated with the indicated drugs for 24 h before methanol fixation. Cells were stained for detyrosinated tubulin (red) and total tubulin (green) and visualised using confocal microscopy (scale bar is 20 µm). For each cell line, fluorescence intensity of detyrosinated tubulin in individual cells was measured using ImageJ and normalised to total tubulin intensity. n = 8 (from two independent experiments), mean values ± SD are shown, analysed by one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.01. c Kymographs of EB3-GFP comets in RPE1 cells treated with 2 nM docetaxel and/or 200 nM mebendazole. Left panels show representative images. The right panel shows microtubule assembly speeds measured from 15 cells for each condition as indicated. Data were pooled from two independent experiments. n = 15, mean values ± SD are shown, analysed by one-way ANOVA with Tukey’s multiple comparisons test, ***p < 0.0001.