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. 2020 Feb 18;9(2):26. doi: 10.1038/s41389-020-0205-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a E-cadherin expression decreased, whereas FIR family and BRG1 expressions increased in cancer tissues (Ca) relative to those in adjacent normal tissues revealed by immunohistochemical staining. Case numbers 2832, 1621, and 1664 are listed in Suppl. Table S4. In the non-invasive early stage (IA) of differentiated cancers, the expressions of E-cadherin and FBW7 were decreased. Case number P16-00361 are listed in Suppl. Table S8. b Migration of E-cadherin siRNAs transfected into NUGC4 cells and corresponding control cells was measured by wound-healing assay. P < 0.01 was obtained by Student’s t-test. c FIR family and related protein expressions were examined aftertreatment of HeLa cells withthe FIRsiRNAs. GL2 siRNA was transfected as the negativecontrol. After 48 h of transfection, whole-cell extracts were analyzed by western blotting. Three types of FIR siRNAs were transfected into HeLa cells. Lane 2 is GL2 siRNA control transfection, lane 3 is 20 pmol of total FIR siRNA transfection, lane 4 is 20 pmol of FIRsiRNA transfection, and lane 5 is 20 pmolof FIRΔexon2 siRNA transfection. d E-cadherin expression in HeLa cells. The level of E-cadherin expression was much higher in NUGC4 than that of HeLa cells. Three types of FIR siRNAs were transfected into NUGC4 and HeLa cells. GL2 siRNA is internal control. 20 pmol of total FIR siRNA, 20 pmol of FIR siRNA, and 20 pmol of FIRΔexon2 siRNA transfection were performed. After 48 h of transfection, whole-cell extracts were analyzed by western blotting.