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qRT-PCR analysis for RNA expression. Gene expression for MMP8 and TBX3 (a) including malat-1 and CRNDE lncRNA (b) were analyzed by qRT-PCR in the same serum exosomes used in EXO-NGS studies. Two biological replicates were used for each normal, IPMN and PDAC samples and the experiments were performed in duplicates. The dissociation curves for the qRT-PCR are shown in supplementary (Fig. 8).
