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. 2020 Feb 12;11:215. doi: 10.3389/fimmu.2020.00215

Figure 5.

Figure 5

OxPAPC treatment resulted in reduced intraocular bacterial load during Bacillus endophthalmitis. (A) Experimental design of in vivo inhibition of TLR2/4 activation. (B) C57BL/6J mouse eyes were infected with 100 CFU WT or ΔslpA B. thuringiensis. At 4 h postinfection WT infected eyes were treated with 30 ng/μL OxPAPC. At 10 h postinfection eyes were harvested, homogenized, and analyzed for bacterial growth. Compared to untreated WT-infected C57BL/6J mouse eyes, a significant reduction in bacterial burden was observed in the OxPAPC-treated group at 10 h postinfection. No difference in intraocular bacterial count was observed between WT and ΔslpA infected eyes at 10 h postinfection. Values represent mean ± SEM of log10 CFU/eye of N ≥ 5 eyes for at least two separate experiments. (C) The in vitro growth of WT B. thuringiensis in BHI was not affected by the presence of 0.1, 1, or 10 μg/mL OxPAPC. Values represent the mean ± SEM of N = 3; multiple comparison, 2-way ANOVA (D) Treatment of eyes with OxPAPC 4 h after infection with 100 CFU of WT B. thuringiensis resulted in decreased bacterial growth. Data represent the mean ± SEM of log10 CFU/eye of N ≥ 5 eyes per time point for at least two separate experiments; ns: P > 0.05, *P < 0.05 at all time points.