Figure 5.

JQ1 regulates PI3K/AKT signalling pathway in GIST. A, GIST48 and GIST430 cell lines were treated with JQ1. Indicated protein level was analysed by Western blotting. B, GIST48 and GIST430 cell lines were transfected with 10 μmol/L JQ1 or 10 μmol/L I‐BET‐151. KIT transcript was analysed. C, GIST48 and GIST430 cell lines were transfected with 10 μmol/L JQ1 or 10 μmol/L I‐BET‐151. KIT promoter activity was analysed. D, GIST48 and GIST430 cell lines were treated with I‐BET‐151. Indicated protein level was analysed by Western blotting. E, GIST48 and GIST430 cell lines were treated with JQ1, imatinib, or their combination. Indicated protein level was analysed by Western blotting. F, GIST430 cell lines transfected with Myr‐AKT were treated with JQ1. Indicated protein level was analysed by Western blotting. *P < .05; **P < .01