Figure 3.

circ‐CCND1 regulates the stability of CCND1 mRNA. A, B, The mRNA expression and protein expression of CCND1 in control and circ‐CCND1‐silenced Hep‐2 and TU212 cells were detected by qRT‐PCR and Western blot assays, respectively. C, qRT‐PCR analysis for the different part of CCND1 mRNA in control and circ‐CCND1‐silenced Hep‐2 and TU212 cells. D, qRT‐PCR analysis for CCND1 mRNA expression in control and circ‐CCND1‐silenced Hep‐2 and TU212 cells at the indicated time after treatment with 1 mg/mL actinomycin D. E, qRT‐PCR analysis for CCND1 mRNA expression in 48 matched LSCC and normal tissues. F, The correlation between circ‐CCND1 and CCND1 mRNA expression in 48 LSCC tissues. G, The proliferative capabilities of circ‐CCND1‐silenced Hep‐2 and TU212 cells after transfection with CCND1 expression plasmid. ** P < .01, *** P < .001