Table 2.
Primer sequences used in this study
Gene | Direction | Sequence (5′‐3′) |
---|---|---|
hsa_circ_0023303 | Forward | TCCTCTCCAAAATGCCAGAG |
Reverse | ACTCTGCTGCTCGCTGCTAC | |
hsa_circ_0023304 | Forward | GCGGAGGAGAACAAACAGAT |
Reverse | AGGAAGCGGTCCAGGTAGTT | |
hsa_circ_0023305 | Forward | TTCCCAGCACCAACATGTAA |
Reverse | AGGAAGCGGTCCAGGTAGTT | |
CCND1 | Forward | AACTACCTGGACCGCTTCCT |
Reverse | TCGGTGTAGATGCACAGCTT | |
CCND1‐5′‐UTR | Forward | CTGGAGCCTCCAGAGGGCTGT |
Reverse | GCGCTCCCTCGCGCTCTTC | |
CCND1‐CDS | Forward | ACGAAGGTCTGCGCGTGTT |
Reverse | CCGCTGGCCATGAACTACCT | |
CCND1‐3′‐UTR | Forward | GGAAAGCTTCATTCTCCTTGTTG |
Reverse | TCTTTTGCTTAAGTCAGAGATGGAA | |
CCND1‐Intron 1 | Forward | CTTTGTTCAAGCAGCGAGTC |
Reverse | AAGGTCCTCCAAGCCGATA | |
CCND1‐Intron 2 | Forward | CCCAGCTCCCTTGAGTCC |
Reverse | CGGTCCTGGATGTTGGAG | |
CCND1‐Intron 3 | Forward | TTTGTCATCGGCCAGAAATA |
Reverse | GACCTTCAGAGCACAGACCA | |
Actin | Forward | GACCTGACTGACTACCTCATGAAGAT |
Reverse | GTCACACTTCATGATGGAGTTGAAGG | |
miR‐646 | Forward | GAAGCAGCTGCCTCTGAGC |
Reverse | CAGAGCGCCAGCGAGGAGCC | |
U6 | Forward | GCTTCGGCAGCACATATACTAAAAT |
Reverse | CGCTTCACGAATTTGCGTGTCAT |