Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 18;10:2815. doi: 10.1038/s41598-020-59736-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression of CAR1 and CD16 in NK92MI cells by RT-PCR and ELISA. (A–C) Total RNA was extracted by Trizol reagents from NK92MI/fCD16 cells stably transfected with CAR1 monomer (labeled as M in panels (A–C) and NK-CAR1 monomer in panel (D) or dimer (labeled as D in panels (A–C) and NK-CAR1 dimer in panel (D). Untransduced parental NK92MI cells were used as a negative control (C) for CAR1 and CD16. (A) CAR1. (B) CD16. (C) Beta actin was assayed as a loading control for total RNAs. Representative photos from two independent experiments. Quick-Load 2-Log DNA ladder (New England Biolabs) was used as DNA markers (0.1–10 Kilobases, Kb). (D) Sandwich ELISA assay using paired anti-human fVII antibodies (Cedarlane Laboratories) for CAR1 expression in RIPA whole cell lysates. NK-GFP: Lentivirus-GFP stably transduced NK92MI/fCD16 cells. Data in (D) were presented as mean ± SEM.