Fig. 2. Azoramide alleviates apoptosis of PLA2G6^D331Y/D331Y DA neurons.

a Representative bright field view of PLA2G6^WT/WT and PLA2G6^D331Y/D331Y DA neurons at days 15 and 20. b Cell viability of PLA2G6^WT/WT and PLA2G6^D331Y/D331Y after 15 and 20 days differentiation culture was measured by CCK-8 assay. c Structure of azoramide. d PLA2G6^D331Y/D331Y neurons at day 15 were treated with azoramide (0.0625, 0.125, 0.25, 0.5, 1 M) or 0.1% DMSO (vehicle control) for 24 h and cell viability was measured using the CCK-8 assay. e Schematic diagram of drug treatment process. f PLA2G6^D331Y/D331Y DA neurons were treated with azoramide (0.1, 0.3, 1, 3, 10 μM) from day 15 to 20, and viability was determined by CCK-8 assay. g, h Expression of apoptosis-related proteins, including cleaved caspase 3, cytochrome C, Bax, Bcl2 and CREB, was detected and quantified by western blotting using specific antibodies. α-Tubulin was the internal control. Data are represented as mean ± SEM. All experiments were replicated in triplicate independently. *p < 0.05, **p < 0.01, ***p < 0.005. Scale bar: 50 µm.