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. 2020 Feb 18;11(2):130. doi: 10.1038/s41419-020-2312-8

Fig. 6. CREB inhibitor blocks the effects of azoramide in PLA2G6D331Y/D331Y neurons.

Fig. 6

a, b PLA2G6D331Y/D331Y DA neurons were treated with 10 μM azoramide or 1 μM CREB inhibitor 666-15, or both, from day 15 to 20. Expression of apoptosis-related proteins was determined and quantified by western blotting. c, d Expression of mitochondrial fission- and fusion-related proteins. e, f Expression of ER-stress-related proteins. g Mitochondrial membrane potential was determined by flow cytometry after JC-1 staining. Data are represented as mean ± SEM. All experiments were replicated in triplicate independently. *p < 0.05, **p < 0.01, ***p < 0.005.