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. 2019 Dec 16;122(4):506–516. doi: 10.1038/s41416-019-0673-5

Fig. 3. MEK inhibition decreases ERK phosphorylation and DUSP4 expression in BRAF wild-type melanoma cell lines.

Fig. 3

a Endogenous expression of DUSP4 and ETV4 protein in duplicate whole-cell extracts of CHL-1 and SK-MEL-23 cells analysed by western blotting. Similar results were obtained in n = 3 independently prepared lysates. A faint ~40-kDa band (arrowhead) just below DUSP4 band was not reduced by DUSP4 depletion (see Fig. 4b, c) so it may be non-specific. b DUSP4 and ETV4 mRNA quantified by qRT-PCR (n = 3 independently prepared cDNAs). c CHL-1 and SK-MEL-23 cells were treated with 100 nM selumetinib or 10 nM trametinib for 1 h before analysis by western blot. Supplementary Fig. 1A–C shows concentration and time dependence of response to MEK inhibition.