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. 2020 Feb 18;11(2):132. doi: 10.1038/s41419-020-2323-5

Fig. 4. Lnc-Lfar1 knockdown suppresses LPS- and IFN-γ-induced proinflammatory activation of macrophages.

Fig. 4

KCs were transfected with lnc-Lfar1 siRNA and following treated with 20 ng/ml LPS or 100 ng/ml IFN-γ for 24 h. a The F4/80 expression was determined by confocal analysis; scale bar = 10 μm. b, c The protein level of CD11b and MCP-1 was determined by western blot. GAPDH was used as an internal control. d, e The RNA level of lnc-Lfar1, F4/80, Ly6c, Tnf-α, Mcp-1, Il-1β, Il-6, iNos, Ccl5 and Cxcl10 was determined by qRT-PCR. *p < 0.05 vs si-NC, #p < 0.05 vs si-NC + LPS/IFN-γ.