. 2020 Feb 18;10:2812. doi: 10.1038/s41598-020-59686-w

Table 1.

Volatile constituents identified from the hydrodistilled (HD), nanocapsules and nanoemulsion of O. glandulosum essential oil using GC-MS.

S/N	Compound	KI^a	% Area^b Origanum glandulosum Desf. oil			Identification method^c,d
S/N	Compound	KI^a	HD	Nanoemulsion	Nanocapsules	Identification method^c,d
1	α-Thujene	928	1.43 ± 0.12	n.d.	1.08 ± 0.07	MS, KI, & ST
2	α-Pinene	932	1.13 ± 0.05	1.02 ± 0.03	0.79 ± 0.06	MS, KI, & ST
3	Camphene	971	0.12 ± 0.03	2.36 ± 0.06	1.13 ± 0.04	MS & KI
4	β–Pinene	978	0.20 ± 0.12	n.d.	n.d.	MS & KI
5	β–Myrcene	991	1.45 ± 0.13	n.d.	2.22 ± 0.06	MS, KI, & ST
6	α-Phellandrene	998	0.21 ± 0.04	n.d.	0.33 ± 0.08	MS & KI
7	Δ–3-Carene	1001	0.10 ± 0.06	n.d.	2.63 ± 0.08	MS & KI
8	α-Terpinene	1004	3.02 ± 0.08	n.d.	n.d.	MS, KI, & ST
9	p-Cymene	1008	11.67 ± 0.33	2.86 ± 0.06	9.09 ± 0.20	MS, KI, & ST
10	Limonene	1029	n.d.	0.72 ± 0.07	0.56 ± 0.04	MS & KI
11	γ-Terpinene	1088	23.43 ± 0.07	1.35 ± 0.08	15.26 ± 0.14	MS, KI,& ST
12	Linalool	1089	0.37 ± 0.05	0.73 ± 0.07	1.8 ± 0.30	MS & KI
13	Camphor	1141	n.d.	1.31 ± 0.13	n.d.	MS & KI
14	Borneol	1148	0.10 ± 0.07	34.29 ± 0.41	n.d.	MS & KI
15	Terpinen-4-ol	1155	0.30 ± 0.06	2.42 ± 0.17	0.65 ± 0.08	MS & KI
16	α-Terpineol	1165	0.49 ± 0.10	35.68 ± 0.42	0.62 ± 0.22	MS & KI
17	Thymol methyl ether	1231	0.19 ± 0.07	n.d.	n.d.	MS & KI
18	Carvacrol methyl ether	1240	0.24 ± 0.08	n.d.	n.d.	MS & KI
19	Thymol	1267	19.52 ± 0.27	13.47 ± 0.26	31.81 ± 0.35	MS, KI, & ST
20	Carvacrol	1276	26.29 ± 0.15	1.93 ± 0.16	28.45 ± 0.18	MS, KI, & ST
21	β–Cubebene	1385	0.33 ± 0.05	n.d.	n.d.	MS & KI
22	β–Caryophyllene	1414	1.65 ± 0.32	0.82 ± 0.10	1.33 ± 0.11	MS, KI,& ST
23	α-Humulene	1451	0.72 ± 0.17	n.d.	n.d.	MS & KI
24	Germacrene D	1472	1.12 ± 0.08	n.d.	n.d.	MS, KI, & ST
25	Bicyclogermacrene	1489	0.14 ± 0.03	n.d.	n.d.	MS & KI
26	α-Muurolene	1491	0.06 ± 0.04	n.d.	n.d.	MS & KI
27	β–Bisabolene	1502	0.52 ± 0.07	n.d.	0.72 ± 0.07	MS & KI
28	γ-Cadinene	1509	1.27 ± 0.09	n.d.	n.d.	MS, KI, & ST
29	γ-Bisabolene	1511	0.29 ± 0.07	n.d.	0.36 ± 0.04	MS & KI
30	Spathulenol	1561	0.16 ± 0.08	n.d.	n.d.	MS & KI
31	Caryophyllene oxide	1576	0.23 ± 0.08	n.d.	n.d.	MS & KI
32	α–epi- Muurolol	1638	0.71 ± 0.08	n.d.	n.d.	MS & KI
33	Torreyol	1641	0.14 ± 0.03	n.d.	n.d.	MS & KI
34	α-Cadinol	1649	1.44 ± 0.10	n.d.	n.d.	MS, KI, & ST
	Total	—	99.04	98.96	98.50	—

^aConfirmed by comparison with Kovat’s index on a DB5 column (Adams 2007).

^bValues represent averages ± standard deviations for triplicate experiments.

^cConfirmed by comparison with the mass spectrum of the authentic compound.

^dIdentification by comparison with data obtained from the NIST mass spectra library.

n.d: not detected.