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. 2020 Feb 18;11(2):131. doi: 10.1038/s41419-020-2326-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a. Elastin structure of aortic wall was evaluated at indicated time points using Weigert’s resorcin-fuchsin elastic staining. Saline treated group was used as control (mock) (left). Elastin degradation was prevented in AngII⁺ BP-1-102⁺ group (AngII⁺BP⁺, lower panel) compared with AngII⁺ group (upper panel) at each time point. b. Mean aortic wall elastin volume of AngII⁺ group (red column) and AngII⁺ BP-1-102⁺ group (AngII⁺BP⁺, blue column) were analyzed at indicated time points. Saline treated group was used as control (green column). Comparing with AngII⁺ group, the AngII⁺ BP-1-102⁺ group showed preserved elastin volume at each time point. The results were presented as mean ± standard deviation (SD). n = 8. *P < 0.01 vs. AngII⁺ group (two-way ANOVA followed by Tukey’s test). NS indicates non-significant differences compared with AngII⁺ group. c mRNA expression of MMP-2 was determined using real-time PCR. Fold changes were calculated and statistically analyzed at indicated time points. Saline treated group were used as control (green column). The results were presented as mean ± standard deviation (SD). n = 8. *P < 0.01 vs. AngII⁺ group (two-way ANOVA followed by Tukey’s test). NS indicates non-significant differences compared with AngII⁺ group. d mRNA expression of MMP-9 was determined using real-time PCR. Fold changes were calculated and statistically analyzed at indicated time points. Saline treated group were used as control (green column). The results were presented as mean ± standard deviation (SD). n = 8. *P < 0.01 vs. AngII⁺ group (two-way ANOVA followed by Tukey’s test). NS indicates non-significant differences compared with AngII⁺ group.