TABLE 5.
Primer pairs used for quantitative PCR
| Target taxa | Primersa | Amplicon size (bp) | Reference |
|---|---|---|---|
| Domain Bacteria (targets V4 region) | F: TGCCAGCMGCCGCGGTAA | 254 | 49 |
| R: GGACTACHVGGGTWTCTAAT | |||
| Clostridium | F: TGCCAGCMGCCGCGGTAA | 131 | 65 |
| R: GGACTACHVGGGTWTCTAAT | |||
| Enterobacteriaceae | F: GTGCCAGCMGCCGCGGTAA | 429 | 66 |
| R: GCCTCAAGGGCACAACCTCCAAG | |||
| “Candidatus Savagella” | F: GATGCGTAGGCGGTTGAGTA | 90 | This study |
| R: GGGTTTCTAATCCTGTTTGCTCC | |||
| Clostridium cluster IV | F: TTACTGGGTGTAAAGGG | 580 | 67 |
| R: TAGAGTGCTCTTGCGTA | |||
| Clostridium cluster XIV (a and b) | F: AAATGACGGTACCTGACTAA | 438–441 | 68 |
| R: CTTTGAGTTTCATTCTTGCGAA |
a
F, forward; R, reverse.