FIG 2.

The content of ROS and H₂O₂ in mycelia can be regulated by the addition of exogenous NO. (A) Change in ROS levels with 100 μM SNP and 250 μM cPTIO treatment, as detected by DCFH-DA staining. (B) Changes in the ROS fluorescence ratio with 100 μM SNP and 250 μM cPTIO. (C) H₂O₂ content with 100 μM SNP and 250 μM cPTIO. H₂O₂ was calculated according to the protein concentration, and gprot is protein amount (in g). The values are the mean ± SE of three independent experiments. Different letters indicate significant differences for the comparison of samples (P < 0.05 according to Duncan’s test).