TABLE 1.
Primers used in this study
| Primera | Sequence (5′→ 3′) | Note |
|---|---|---|
| Po-gpd-F | GGTACCTTTATTGGCGGT | Promoter cloning |
| Po-gpd-R | CCAGGTCAGTGAAATTTCC | |
| aco_g-F | ATGTTCAACTGCGACCG | gDNA fragment cloning |
| aco_g-R | CTAGTTGCCCTTGGCCTTAGC | |
| aco_c-F | ATGTTCAACTGCGACCG | cDNA fragment cloning |
| aco_c-R | CTAGTTGCCCTTGGCCTTAGC | |
| aox_g-F | ATGCTTCGCGCGCAACTC | gDNA fragment cloning |
| aox_g-R | TCAATTACTACCTTTCTGCTTCTCT | |
| aox_c-F | ATGCTTCGCGCGCAACTC | cDNA fragment cloning |
| aox_c-R | TCAATTACTACCTTTCTGCTTCTCT | |
| aco-q-F | CCTCACCGTTCTCAATGTT | qPCR |
| aco-q-R | CGACGAAGGCATGAGTAG | |
| aox-q-F | CTCCACAGATCCACACTCTTC | |
| aox-q-R | CCGCCTCAAAGTCATAAAAGTC | |
| β-actin-F | GCGATGAACAATAGCAGGG | Endogenous control |
| β-actin-R | GCTGGTATCCACGAGACAAC | |
| aco-OE-F | TTACAGGTCAAAGTTATGTTCAACCTAGGAACCG | Construction of OE plasmids |
| aco-OE-R | AATTCTAGAGG GCCCCTAGTTGCCCTTGGCCTTAGC | |
| aox-OE-F | GGTCAAAGTTACTAGTATGCTTCGCGCGCAACTC | |
| aox-OE-R | CAATTCTAGAGGGCCCTCAATTACTACCTTTCTGCTTCTC | |
| aco-RNAi-F1 | CTTTACCATCTCCTCAGATCTAACTTGTCAGAACTCGAGCCACA | Construction of RNAi plasmids |
| aco-RNAi-R1 | GGAGCTAAGCTCTAAACTAGTCTGCGAGTGACTCGGTGAGC | |
| aco-RNAi-F2 | TTCTTCTATTATAAGACTAGTTAGAGTTGGGTTCGCCCTTCT | |
| aco-RNAi-R2 | CATGCCAATTCTAGAGGGCCCAACTTGTCAGAACTCGAGCCACA | |
| gpd+aco-F | CTACTACTCCTTGACCGCTGAT | Detection of transformants |
| gpd+aco-R | AATCCTTGACACCACCAACTTG | |
| hyg-F | CGACAGATCCGGTCGGCATCTACTCTATTTCTT | |
| hyg-R | TCTCGTGCTTTCAGCTTCGATGTAGGAGGG | |
| gapdh-F | ATCCATATCTTCGCTGAGAA | |
| gapdh-R | GGACGGAATAATGTTGTTGT |
a
F, forward; R, reverse.