FIG 1.

(A) Putative methyltransferase domain sequences of A. nidulans (Anid) LaeA, A. niger (Anig) LaeA, and predicted A. kawachii (Akaw) LaeA-like proteins. The domains were identified by Pfam (https://pfam.xfam.org/). Sequence alignment was performed using ClustalW program in the BioEdit Sequence Alignment Editor (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). Putative intron sequences of A. kawachii laeA and laeA2 were confirmed by transcriptome sequencing (RNA-seq; data not shown) and translated to the amino acid sequences. Asterisks indicate putative SAM binding sites (11). (B) Phylogenetic tree of amino acid sequences of LaeA-like methyltransferases identified from the Aspergillus genome database (http://www.aspgd.org/). The tree was constructed via the neighbor-joining method with complete gap deletion on MEGA version 6.0 (53). Bootstrap values (1,000 replicates) are indicated at the branches. Low bootstrap values (<25) were removed.