Figure 2.

A. rhizogenes-mediated induction of transgenic hairy roots and selection of optimal infection conditions. (a) Chimeric callus tissues induced from hypocotyl explants. (b–d) Transgenic hairy roots from hypocotyl explants three, four, and six weeks after infection, respectively. (e) Chimeric callus tissues induced from leaf explants. (f–h) Transgenic hairy roots from leaf explants three, four, and six weeks after infection, respectively. (i) Validation of transgenic hairy roots using PCR analysis, the full-length gels were shown in Figure S2(a–e). (j) Co-transformation rate of various infection concentrations (OD_600nm) with 30 min infection duration. (k) Co-transformation rate of various infection durations (min) with infection OD_600nm = 0.8. (l) Co-transformation rate of various varieties (30 min infection duration and OD_600nm = 0.8). The left and right images in a-h were captured using bright field and fluorescence microscopy, respectively, with a scale bar of 1 cm. Hypocotyl was the infection explant in (j–l), different lowercase letters in (j–l) indicate statistically significant difference (P < 0.05).