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. 2020 Feb 18;10:2860. doi: 10.1038/s41598-020-59529-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alpha and beta diversity comparisons of microbiomes collected from CRC patients and healthy controls. Analysis was performed using sequencing data for the 16S rDNA V3 and V4 regions, with a rarefaction depth of 10,000 reads per sample. (A) Chao1 and Shannon indexes indicate alpha diversity. Whiskers in the boxplots represent the range of the minimum and maximum alpha diversity values within a population, excluding outliers. Principal coordinate analysis (PCoA) plots represent beta diversity at the (B) phylum and (C) genus levels. Red circles represent healthy control individuals and blue circles represent CRC patients. CRC, colorectal cancer.