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. 2020 Feb 18;10:2851. doi: 10.1038/s41598-020-59469-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DHE and JQ1 suppress transcription progression across the MYC locus. (A) Dose responsive expression of NR4A1, NR4A3 and MYC mRNAs using RT-qPCR in MOLM-14 cells treated with specified concentrations of DHE or JQ1 for 6 hours. (B) ChIP-qPCR for total RNA polymerase II (Pol 2), Pol 2 phospho serine 5 (p-S5), Pol 2 phospho serine 2 (p-S2), CDK9, and H3K36me3. ChIP-qPCRs were done using primer pairs that span the MYC locus at 4 hours after treatment with 10 uM DHE or 500 nM JQ1. ***p < 0.001, **p < 0.01, *p < 0.05 compared to vehicle controls.