Figure 6.
DHE and JQ1 suppress tumor growth and intratumoral MYC expression in a xenograft mouse model of MLL-rearranged AML. (A) Schematic depicting subcutaneous xenograft strategy. (B) 7 week-old NSG mice were transplanted with 1 × 107 MV4–11 cells, and monitored until average tumor volume reached 60 mm3, at which point intraperitoneal injections of vehicle, 4.0 mg/kg DHE, 30 mg/kg JQ1, or the two drugs combined were administered twice daily. Average tumor volume is indicated. (C) Immunohistochemical staining and quantification of proliferative marker Ki67, and MYC in MV4–11 xenograft tumor tissues. ***p < 0.001, **p < 0.01, *p < 0.05 compared to vehicle controls.