. 2020 Feb 18;10:2835. doi: 10.1038/s41598-020-59707-8

Table 1.

Properties of R-CEPIA3mt and R-CEPIA4mt.

Probe	Ca²⁺	ε (mM⁻¹ cm⁻¹) (λ_ABS*)	pK_a^†	λ_Ex^*	pH 7.2			pH 8.0
Probe	Ca²⁺	ε (mM⁻¹ cm⁻¹) (λ_ABS*)	pK_a^†	λ_Ex^*	K_d for Ca²⁺ (μM)	Dynamic range^‡	Hill coefficient	K_d for Ca²⁺ (μM)	Dynamic range^‡	Hill coefficient
R-GECO1mt	−	27 (445), 7 (576)	8.9	565	0.19 ± 0.02^§	22.2 ± 0.5^§	2.20 ± 0.10^§	0.14 ± 0.01^§	8.8 ± 0.1^§	2.12 ± 0.10^§
R-GECO1mt	+	54 (562)	6.2	565	0.19 ± 0.02^§	22.2 ± 0.5^§	2.20 ± 0.10^§	0.14 ± 0.01^§	8.8 ± 0.1^§	2.12 ± 0.10^§
R-CEPIA3mt	−	26 (445), 6 (576)	8.9	565	3.7 ± 0.5	30.0 ± 1.5	0.96 ± 0.02	3.3 ± 0.2	8.9 ± 0.1	0.93 ± 0.01
R-CEPIA3mt	+	49 (562)	6.5	565	3.7 ± 0.5	30.0 ± 1.5	0.96 ± 0.02	3.3 ± 0.2	8.9 ± 0.1	0.93 ± 0.01
R-CEPIA4mt	−	25 (445), 6 (576)	8.8	565	26.9 ± 1.0	23.9 ± 0.4	1.51 ± 0.01	21.4 ± 0.3	4.7 ± 0.1	1.86 ± 0.02
R-CEPIA4mt	+	17 (450), 30 (562)	6.5	565	26.9 ± 1.0	23.9 ± 0.4	1.51 ± 0.01	21.4 ± 0.3	4.7 ± 0.1	1.86 ± 0.02

*λ_ABS and λ_Ex are the maximum wavelength of absorption and fluorescence excitation spectra, respectively.

^†pKa is determined as the pH at half-maximal fluorescence intensity calculated by fitting Hill equation to each plot.

^‡Dynamic range indicates the ratio of the maximum to minimum fluorescence intensity (F_max/F_min).

^§Mean ± s.e.m.