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. 2020 Feb 18;10:2845. doi: 10.1038/s41598-020-59444-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of StarD7 during myogenic differentiation in C2C12 cells. (a) The protein levels of StarD7, MYH4, MYH6, and myogenin in C2C12 cells cultured in differentiation medium for the days indicated were analyzed by western blotting. GAPDH was used as a protein loading control. (b) The mRNA levels of StarD7 and myogenin in C2C12 cells differentiated for the days indicated were quantified by qPCR. Values shown are means ± S.D. ***P < 0.001 as compared with day 0. ns indicates not significant as compared with day 0 (one-way ANOVA with Tukey’s post hoc test).