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. 2020 Feb 18;10:2845. doi: 10.1038/s41598-020-59444-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The loss of StarD7 impaired myogenic differentiation in C2C12 cells. (a) After transfection with siRNAs against StarD7 (KD1 or KD2) or control siRNA, C2C12 cells were cultured in differentiation medium for 5 days, then immunostained with anti-MYH6 antibody (green). Nuclei were stained with DAPI (blue). Bars indicate 50 μm. (b) Protein levels of MYH4, MYH6, myogenin and StarD7 were analyzed by western blotting. GAPDH was used as a protein loading control. (c) The mRNA levels of MYH4, MYH6, myogenin, myomaker, myomerger (L), myomerger (S) and PGC-1α were quantified by qPCR. Data were normalized to the GAPDH level. Values shown are means ± S.D. from three independent culture dishes. ***P < 0.001 as compared with control siRNA (one-way ANOVA with Tukey’s post hoc test). (d) Protein levels of StarD7 in WT and StarD7-KO C2C12 cells were analyzed by western blotting. (e) WT or StarD7-KO C2C12 cells were cultured in differentiation medium for 5 days and phase contrast images were obtained.