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. 2020 Feb 18;10:2845. doi: 10.1038/s41598-020-59444-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reintroduction of StarD7 into the StarD7-KO C2C12 cells restored mitochondrial PC composition. Mitochondria were purified from WT + EV, KO + EV, and KO + StarD7 C2C12 cells, phospholipids were extracted, and the amounts of (a) PC and (b) PE were determined using LC-MS/MS with multiple reaction monitoring. Values were normalized against the amount of mitochondrial protein. The results shown are from one representative experiment of five experiments giving similar results. Data are means ± S.D. from quadruplet analyses of one experiment. **P < 0.01 and ***P < 0.001 as compared with WT + EV cells. ^†P < 0.05, ^††P < 0.01, and ^†††P < 0.001 as compared with KO + EV cells (one-way ANOVA with Tukey’s post hoc test).