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. 2020 Feb 18;10:2845. doi: 10.1038/s41598-020-59444-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of StarD7 impaired myogenic differentiation in human primary myoblasts. (a) After transfection with siRNAs against StarD7 (KD) or control siRNA, human primary myoblasts were cultured in differentiation medium for 3 days, then immunostained with anti-MYH6 antibody (green). Nuclei were stained with DAPI (blue). Bars indicate 100 μm. (b) The fusion indexes were calculated, and are presented as the means ± S.D. ***P < 0.001 as compared with control siRNA (Student’s t test). (c) After inducing differentiation, protein levels of MYH4, MYH6, myogenin and StarD7 were analyzed by western blotting. GAPDH was used as a protein loading control. (d) The mRNA levels of myogenin, myomaker, myomerger and PGC-1α were quantified by qPCR after inducing differentiation. Data were normalized to the GAPDH level. Values shown are means ± S.D. from three independent culture dishes. ***P < 0.001 as compared with control siRNA (Student’s t test).