Table 1.

Primers used in this study

Fungus	Gene target	Primer name	Primer orientation	Primer sequence (5′−3′)	Expected band size (bp)	Reference
C. gloeosporioides sensu lato	Species complex‐specific ITS	CgInt	Forward	GGCCTCCCGCCTCCGGGCGG	450	Mills, Sreenivasaprasad, and Brown (1992)
		ITS4	Reverse	TCCTCCGCTTATTGATATGC		White et al. (1990)
Fungi	Fungi ITS	ITS5	Forward	GGAAGTAAAAGTCGTAACAAGG	550–650	White et al. (1990)
		ITS4	Reverse	TCCTCCGCTTATTGATATGC		White et al. (1990)
C. truncatum	Species‐specific ITS	Ccap‐F	Forward	GTAGGCGTCCCCTAAAAAGG	394	Torres‐Calzada et al. (2011)
		Ccap‐R	Reverse	CCCAATGCGAGACGAAATG
C. gloeosporioides sensu lato	Cutinase	CgCUT‐F	Forward	ATCAGGGTCAGCTAGGTTAGT	308	This study
		CgCUT‐R	Reverse	GGATCGTGAGGCCCTATTTATG
C. truncatum	Cutinase	CcCUT‐F	Forward	AGAGTTTCTTCCGACCATTCC	729	This study
		CcCUT‐R	Reverse	GCCCTGTTATAGGAGTCAGTTATC
C. gloeosporioides sensu lato	partial Actin	ACT−512	Forward	ATGTGCAAGGCCGGTTTCGC	300	Carbone and Kohn (1999)
		ACT−738	Reverse	TACGAGTCCTTCTGGCCCAT
Filamentous Ascomycetes	partial β‐Tubulin	Bt2a	Forward	GGTAACCAAATCGGTGCTGCTTTC	170	Glass and Donaldson (1995)
		Bt2b	Reverse	ACCCTCAGTGTAGTGACCCTTGGC