Table 9.
Comparison between the classification of individuals based on genetics (microsatellites) and shell morphology for each location
| Location | N GEN and GM data | GEN and GM | GEN and GM | Intermediate shape | GEN and GM | GEN Hybrids |
|---|---|---|---|---|---|---|
| Littorina fabalis | Littorina obtusata | Mismatch | GM assigned to species | |||
| N (%) | N (%) | N (%) | N (%) | N (species) | ||
| Burela | 12 | 11 (91.67) | — | 0 | 1 (8.33) | |
| Abelleira | 16 | 6 (40.00) | — | 5 (33.33) | 4 (26.67) | 1 (L. obtusata) |
| Muros North | 14 | 6 (60.00) | 2 (50.00) | 2 (14.29) | 4 (28.57) | |
| Muros Southa | 18 | 17 (94.44) | — | 1 (5.56) | 0 | |
| Lanzada North | 19 | — | 14 (73.68) | 3 (15.79) | 2 (10.53) | |
| Lanzada Southa | 14 | 12 (85.71) | — | 2 (14.29) | 0 | |
| Seixiños | 37 | 16 (64.00) | 3 (27.27) | 8 (22.22) | 9 (25.00) | 1 (L. fabalis) |
| Aldán North | 22 | — | 6 (28.57) | 11 (52.38) | 4 (19.05) | 1 (L. obtusata) |
| Aldán South | 8 | 2 (50.00) | 4 (100) | 1 (12.50) | 1 (12.50) | |
| Borna | 21 | 4 (26.67) | 4 (66.67) | 5 (23.81) | 8 (38.10) | |
| Tirán | 32 | 26 (81.25) | — | 3 (9.38) | 3 (9.38) | |
| Cangas | 25 | 10 (62.50) | 6 (66.67) | 4 (16.00) | 5 (20.00) | |
| Redondelaa | 36 | — | 35 (97.22) | 1 (2.78) | 0 | |
| La Guia | 32 | 4 (33.33) | 17 (85.00) | 5 (15.63) | 6 (18.76) | |
| Alcabre | 22 | 2 (40.00) | 16 (94.12) | 3 (13.64) | 1 (4.55) | |
| Canidoa | 18 | 18 (100) | — | 0 | 0 | |
| Viana do Casteloa | 36 | — | 36 (100) | 0 | 0 | |
| Rio de Moinhosa | 28 | — | 25 (89.29) | 3 (10.71) | 0 | |
| Póvoa de Varzima | 16 | 16 (100) | — | 0 | 0 | |
| Mindelo | 42 | 26 (100) | 13 (86.67) | 0 | 2 (4.88) | 1 (L. obtusata) |
| Agudelaa | 18 | 18 (100) | — | 0 | 0 | |
| Cabo do Mundo | 37 | 11 (100) | 6 (42.86) | 3 (12.00) | 5 (20.00) | 2 (L. obtusata); 10 (L. fabalis) |
| Leça da Palmeiraa | 33 | 32 (96.97) | — | 1 (3.03) | 0 | |
| Total | 556 | 237 | 187 | 61 | 55 | 16 |
Shown is: N GEN and GM data, the number of individuals analyzed for both genetics and shell morphology; GEN and GM L. fabalis N (%), number (and percentage) of concordant assignment using genetics and shell morphology to L. fabalis; GEN and GM L. obtusata N (%), number (and percentage) of concordant assignment using genetics and shell morphology to L. obtusata; Intermediate shape N (%), number (and percentage) of individuals genetically pure with intermediate shell shape; GEN and GM Mismatch N (%), number (and percentage) of individuals genetically classified into one species with shell shape typical from the other species; GEN Hybrids, number of individuals genetically hybrid classified as pure from each species based on shell morphology.
For the GM analysis, allopatric sites were analyzed separately from the sympatric ones (see Section 2).