Figure 2.

(a) Environmental DNA protocol sampling design to test effects of various sampling methods in streams. Sampling took place at seven different sites along a transect. We created two samples at each site with 2 different volumes: 1 L and 2 L. All five sites were visited at four different sampling occasions over the summer from May to August of 2016. (b) eDNA protocol sampling design to test effects of various sampling methods in lakes. Sampling consisted of combining five consecutive points, spaced 50 m apart. Each point contributed equal amounts of water to create two samples of two different target volumes: 1 L and 2 L. All five sites were visited at four different sampling occasions over the summer from May to August in 2016