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Environmental DNA primer and probe sequences for Rana sierrae and Rana boylii quantitative PCR assays. Bold indicates a base pair difference between the species
| Species | Primer/probe | Sequence |
|---|---|---|
|
R. sierrae 88 bp |
RASI_F | CCTTAGCGGGCCTTCCA |
| RASI_R | GCAAGTAGAGTTGCGTTTTGTTTAAT | |
| RASI‐Probe | JUN‐CCTCACAGGCTTCGCTCCCAAACTC‐QSY | |
|
R. boylii 93 bp |
RABO_F | TCCGCCTCATGATCGAAAAC |
| RABO_R | GGCGAAGCCTGTGAGR | |
| RABO‐Probe | 6FAM‐CCCTTTCCACAACCACTA‐MGB |
