Figure 6.

The effect of [U-¹³C₆]glucose concentration on metabolic fluxes relevant to energy production and the citric acid cycle. A, glycolytic efflux of lactate and pyruvate formed from exogenous [U-¹³C₆]glucose. B, percentage contribution of exogenous [U-¹³C₆]glucose to the intracellular pyruvate pool. C, percentage contribution to acetyl-CoA formation of exogenous [U-¹³C₆]glucose via pyruvate decarboxylation (PDC_glu) relative to citrate synthesis (CS). D. Percent contribution of exogenous [U-¹³C₆]glucose to anaplerosis via pyruvate carboxylation (PC_glu) relative to CS. E, ratio of exogenous [U-¹³C₆]glucose undergoing PC_glu to PDC_glu. n = 5 for 5.5 mm and n = 3 for 25 mm. Horizontal bars, means ± S.E. (error bars). *, p < 0.05 between the glucose concentrations. p = 0.08 in B and p = 0.09 in C.