Table 1.
Characteristics of LC-QToF method for (semi)quantitative analysis of metabolites related to the HPB and glycolysis in heart tissues
Intraday and interday reproducibility for the (semi)quantification of metabolites were determined by analyzing eight homogenates of 50 mg of pulverized heart tissues added to 4 nmol of [13C2]UDP-GlcNAc and 150 nmol of [13C5-15N2]glutamine for 3 consecutive days. RT, retention time.
| Analyte | m/z | Internal/external standard | m/z | RT | Intraday %CV | Interday %CV |
|---|---|---|---|---|---|---|
| min | ||||||
| UDP-GlcNAc | 606.0743 | [1,2-13C2]UDP-GlcNAc | 608.0815 | 9.8 | 4.0 | 6.3 |
| Glutamine | 145.0619 | [13C7]glutamine | 152.0727 | 8.8 | 2.7 | 3.7 |
| G6P | 259.0224 | [1,2-13C2]UDP-GlcNAc | 608.0815 | 10.5 | 12.9 | 12.3 |
| F6P | 259.0224 | [1,2-13C2]UDP-GlcNAc | 608.0815 | 10.1 | 8.2 | 9.6 |
| F1,6dP | 338.9888 | [1,2-13C2]UDP-GlcNAc | 608.0815 | 11.9 | 27.3 | 29.9 |