Table 1.

Oxidized phospholipid-apoprotein cross-link adducts identified in HDL3 treated with KOOA-PC

HDL3 (1.5 mg/ml) was incubated with KOOA-PC (10 μm) at 37 °C under the protection of argon. After 4 h incubation, samples were subjected to NaBH₄ reduction, tryptic digestion, peptide enrichment, and LC-MS/MS analysis. The MS/MS spectra obtained were searched against a protein sequence database containing major and minor apoproteins of human HDL using Massmatrix search engine to identify oxPL-peptide cross-link adducts as described under “Experimental procedures.”

HDL apoproteins cross-linked by oxPLs	Cross-linked residues	Peptides cross-linked by oxPLs		na
ApoA-I–apoA-I	AI H135–AI K107	K$WQEEMELYRQKb	LH$ELQEK	3
	AI H155–AI K106	AH$VDALRTHLAPYSDELR	VQPYLDDFQK$K	6
	AI H162–AI K94	DLEEVK$AKVQPYLDDFQKK	TH$LAPYSDELR	6
	AI H162–AI K96	AK$VQPYLDDFQK	TH$LAPYSDELR	3
	AI H162–AI K106	AHVDALRTH$LAPYSDELR	VQPYLDDFQK$K	6
	AI H193–AI K195	LAEYH$AK$ATEHLSTLSEK		4
	AI H199–AI K195	LAEYHAK$ATEH$LSTLSEK		6
	AI H135–AI K182	QRLAARLEALK$ENGGARLAEYHAK	QKLH$ELQEKLSPLGEEMR	6
	AI H155–AI K12	ARAH$VDALRTHLAPYSDELR	VK$DLATVYVDVLK	4
	AI H199–AI K133	AELQEGARQK$LHELQEKLSPLGEEMR	ATEH$LSTLSEK	3
	AI H199–AI K140	ATEH$LSTLSEK	LHELQEK$LSPLGEEMR	5
	AI H193–AI K208	LEALKENGGARLAEYH$AKATEHLSTLSEK	AK$PALEDLR	4
ApoA-I–apoA-II	AI H135–AII K46	AELQEGARQKLH$ELQEKLSPLGEEMR	SYFEKSK$EQLTPLIK	5
	AI H135–AII K30	AELQEGARQKLH$ELQEKLSPLGEEMR	VK$SPELQAEAK	4
	AI H155–AII K28	AH$VDALRTHLAPYSDELRQR	DLMEK$VKSPELQAEAK	4
	AI H193–AII K46	SYFEKSK$EQLTPLIK	LAEYH$AK	5
Minor apoproteins–apoA-I/apoA-II	AI K140–AIV K73	QKLHELQEK$LSPLGEEMR	LAK$DSEKLKEEIGK	6
	AI H162–AIV K73	LAK$DSEKLKEEIGK	TH$LAPYSDELR	4
	AI H162–apoE K233	TRDRLDEVK$EQVAEVRAK	TH$LAPYSDELR	6
	AII K46–AIV K96	SYFEKSK$EQLTPLIK	LLPH$ANEVSQK	6
	AII K44–AIV K227	SYFEK$SKEQLTPLIK	K$NAEELK	4
	AII K46–apoE K157	DADDLQK$RLAVYQAGAR	SK$EQLTPLIK	6
	AI H162–CI K48	TH$LAPYSDELR	EWFSETFQK$VKEKLK	3
	AI H162–CI K37	IKQSELSAK$MREWFSETFQK	TH$LAPYSDELR	2
	AI K96–CIV K26	WSLVRGRMK$ELLETVVNR	AK$VQPYLDDFQKK	3
	AII K30–CIV K81	DLMEKVK$SPELQAEAK	DSLLK$KTHSLCPR	3

^a n represents number of experiments in which specific cross-link adduct was detected.

^b H$ and K$ represent histidine and lysine residues cross-linked by oxPLs. AI, AII, AIV, CI, and CIV represent apoA-I, apoA-II, apoA-IV, apoC-I, and apoC-IV, respectively.