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Identifying genetic modifiers of cellular sensitivity to josamycin in K562 cells through an shRNA screen. A, structures of common macrolide antibacterial agents (erythromycin, clarithromycin, and josamycin) and the nonmacrolide antibacterial chloramphenicol. B, josamycin dose–response in K562 cells after 48 h. Points indicate means ± S.D.; n = 3. C, overview of the shRNA screening process in K562 cells.
