Figure 3.

Testing the relevance of gene hits identified with josamycin across a panel of bacterial ribosome inhibitors. A, comparison of knockdown of MTIF2 on cellular sensitivity to josamycin, clarithromycin, and chloramphenicol after 9 days. The KD/WT ratio is the proportion of knockdown to WT K562 cells in an untreated versus drug-treated population at concentrations of 6, 12.5, and 25 μm after 9 days of coculture initiated at a 1:1 ratio. At these concentrations, all drugs caused decreases in cell growth, as assessed by flow cytometry. B, comparison of knockdown of GPI on cellular sensitivity to various drugs after 9 days. C, comparison of knockdown of MAP3K4 on cellular sensitivity to various drugs after 9 days. D, comparison of the nontargeting shRNA construct (negative control) on cellular sensitivity to various drugs after 9 days. In A–D, error bars indicate mean ± S.D., n = 2, and dots represent individual values.