Skip to main content
. 2020 Jan 9;295(7):2034–2042. doi: 10.1074/jbc.AC119.011795

Figure 1.

Figure 1.

Enhanced expression of thermogenic proteins in iWAT of CD137 knockout mice after cold exposure. A, body weights of WT (open bars) and knockout (solid bars) mouse cohorts kept at room temperature (RT) and after chronic (4-week) cold exposure. B and C, weights of inguinal (B) and epididymal (C) fat depots in WT (open bars) and knockout (solid bars) mouse cohorts kept at RT and after chronic (4-week) cold exposure. D, expression of Ucp1 mRNA assessed by Q-PCR in iWAT of WT and CD137 knockout mice housed at RT or at 8 °C for 4 weeks (cold). Results are expressed as average ± S.E. of -fold change over WT at room temperature, n = 8 mice per group. *, p < 0.05; **, p < 0.01; 2-way ANOVA. E, expression of UCP1 protein assessed by Western blotting in iWAT of WT and CD137 knockout mice housed at RT or at 8 °C for 4 weeks (cold). Results are expressed as average ± S.E. of -fold change over WT at room temperature, n = 8 mice per group. *, p < 0.05; **, p < 0.01; 2-way ANOVA. Representative blots probed for UCP1 and GAPDH are show on the right-hand side. F and G, expression of Cidea (F) and Cox8B (G) mRNAs assessed by Q-PCR in iWAT of WT (open bars) and CD137 knockout (solid bars) mice housed at RT or at 8 °C for 4 weeks (cold). Results are expressed as average ± S.E. of -fold change over WT at RT, n = 8 mice per group. *, p < 0.05; **, p < 0.01; 2-way ANOVA. H, mitochondrial content assessed by Q-PCR for the mitochondrial gene cytochrome B (CytB) normalized to nuclear DNA in iWAT of WT (open bars) and CD137 knockout (solid bars) mice housed at RT or at 8 °C for 4 weeks (cold). Results are expressed as average ± S.E. of -fold change over WT at RT, n = 6 mice per group. *, p < 0.05. I to P, expression of Leptin (I), CD137 (J), CD137L (K), Ifnγ (L), CD301 (M), CD206 (N), Ccl2 (O), and Resistin (P) mRNAs assessed by Q-PCR in iWAT of WT (open bars) and CD137 knockout (solid bars) mice housed at RT or at 8 °C for 4 weeks (cold). Results are expressed as average ± S.E. of -fold change over WT at RT, n = 8 mice per group. *, p < 0.05; 2-way ANOVA. Q and R, expression of Ucp1, Dio2, Pgc1α, and Tfam mRNAs assessed by Q-PCR in brown adipose tissue of WT and CD137 knockout mice housed at room temperature (A) or at 5 °C for 4 weeks (B). Results are expressed as average ± S.E. of -fold change over WT, n = 8 mice per group.