Figure 2.
Binding kinetics of the PSG supramodule and CRIPT. A, example of kinetic transients for the association of PSG with D-CRIPT6. PSG (1 μm final concentration) was mixed rapidly in the stopped flow with D-CRIPT6 at different concentrations. B, the residuals are from fits to single- and double-exponential functions, respectively, at 4 μm final concentration of D-CRIPT6. C, the experiment was repeated over a range of D-CRIPT6 concentrations, and the kobs values obtained from the fit to a double-exponential function were plotted versus [D-CRIPT6]. kobs values of more than 150 s−1 were omitted because they very slightly but progressively deviated from a straight line because of instrumental limitations in the mixing. D, kinetic amplitudes associated with the respective kobs value.