Figure 5.

The relative composition of retained cellular constituents differs between C. neoformans and C. gattii leaky mutant and parent strain melanin ghosts. A, 1D ¹³C DPMAS spectra of melanin ghosts generated from C. neoformans and C. gattii parent and leaky melanin strain cell cultures containing d-[U-¹³C₆]glucose as the sole carbon source. B, measurement of each cellular constituent type relative to the total amount of retained nonpigment moieties obtained from quantitatively reliable ¹³C DPMAS spectra. PolyS, polysaccharide. Each spectrum is normalized by setting the largest peak to full scale. The top of the largest peak was cropped to improve the visibility of smaller peaks.