Figure 6.

C. neoformans H99 melanin ghosts contain highly deacetylated chitosan. 2D ¹³C-¹⁵N TEDOR spectra of C. neoformans H99 melanin ghosts generated from cell cultures containing d-[U-¹³C₆]glucose and [¹⁵N]glycine as the sole carbon and nitrogen sources, respectively. Coherence transfer periods of 1.40 and 4.00 ms were used in separate experiments to obtain proximal carbon-nitrogen pairs separated by distances corresponding to ∼1 bond length (dark purple contours) and ∼2 bond lengths (light purple contours). The boxed numbers displayed on the chitin GlcNAc and chitosan glucosamine (GlcN) monomeric units denote the number of bonds between each ¹³C-¹⁵N nuclear pair.