Figure 12.

Overexpression of AP and localization of human PC7 or its CT mutant with TGN. A, immunofluorescence of hPC7 or its CT mutant (red labeling) in the presence of AP proteins on permeabilized HeLa cells. Cell compartments Golgin-97 marker (TGN marker) are labeled in green. Cell nuclei are marked by DAPI (blue labeling). Quantification, using IMARIS software, of the co-localization between hPC7 (B), and its mutant E719A,E721A,L725A (C) and Golgin-97 in the presence of overexpressed AP-1m (B) or AP-2m (B and C). These results are representative of minimum three independent experiments, and quantification is representative of n = 15 cells. Error bars indicate averaged values ± S.D. ns, not significant (Student's t test). Bar = 1 μm.