Figure 7.

Localization of human PC7 and its CT mutants with early endosomes. Immunofluorescence of hPC7 or its CT mutants (red labeling) on permeabilized HeLa cells. The early endosomal marker (EEA1) is labeled in green. Cell nuclei are marked by DAPI (blue labeling). Quantification of co-localization between hPC7 and its mutants with EEA1 using IMARIS software. These results are representative of a minimum three independent experiments, and quantification represents an average of n = 15 cells. Error bars indicate averaged values ± S.D. **, p < 0.01; ***, p < 0.001 (Student's t test). Bar = 1 μm.